Evolutive multidetection microplate reader

SPECTROFLUOROMETER for 10 STIRRED CUVETTES with 90° geometry

European manufacturer, SAFAS introduced in 1959 the world''s first grating monochromator spectrofluorometer, and has always been a technological leader in spectroscopy : 62 years of breakthroughs, with commitment to performance, reliability and customer''s satisfaction.

Today, these unique experience and know-how are gathered in an outstanding microplate reader, the impressive SAFAS Xenius. Its quality grating monochromators are guaranteed 100%AIR technology (no fiber optics), have ultra-low straylight, 0.2nm accuracy, 0.01nm repeatability and autocalibration on built-in standard, which is unique on the market, enabling innovative analytical techniques, and providing outstanding sensitivity on cuvettes and microplates. Additional fiber optics enable in situ measurements. Why use old-fashioned filters at fixed wavelengths when you can get the highest sensitivity and reproducibility with quality monochromators, automatically at any wavelength, and without any additional filter to purchase ?

The exclusive SAFAS Double Optical Plane Technology eliminates or reduces interferences, exhausts sensitivity and even enables to measure absorbance and fluorescence with compartment opened, allowing easy micropipette injections during measurements at open sky, and providing full protection of the PM tube.

The Xenius is evolutive and can receive 1 to 15 auto-injectors with 0.1µl accuracy and 6-way automatic valves, enabling to inject in cuvettes as well as in microplates, in measurement position; priming and washing are fully automated.

The 10 aligned and stirred cuvettes are obviously read vertically, with 90° beam geometry. Holder is ultra-fast (5 seconds) and stirring is software driven. Cuvettes are precious to finely tune the microplate parameters, and also for the most diluted samples. Micro-injection protocols are possible, as low as 0.1µl per injection during measurements in 250µl stirred samples.

Fluorescence excitation, emission, 3D and synchronous spectra are possible, with instrumental correction to draw absolute fluorescence spectra, and steps as small as 0.1nm. Bandwidths and continuously variable from 1 to 30nm by steps of 0.1nm, independently at excitation and emission, which enables fine tuning of experimental conditions. The continuously variable voltage of the high sensitivity PM tube enables to monitor the weakest signals as well as strong lights (like reflectance, absorbance, turbidity), enabling to achieve innovative measurements and corrections. An ingenious integrating sphere enables easy measurements of quantum yields on solids and powders with just one insertion of the sample and without needing any additional attenuator…

Fluorescence, Bioluminescence and Absorbance can now be measured on 1µl in cuvette, as well as on 96x1µl in microplates. These microplates are re-usable but plastic made, they can be replaced at low cost when necessary.

In Bioluminescence, the amazing dynamic linearity up to 100Mcps and dynamic range of 9 decades are coupled to the highest sensitivity and very low cross-talking, providing an amazing performance and flexibility. Bioluminescence spectra can be drawn up to 1000nm, and high sensitivity BRET measurements are available.

Temperature control is possible in cuvettes as well as in microplates, cooling as well as heating, with ingenious solutions to remove condensation. Thermostatation is homogeneous and maintained even during measurements.

A patented technology enables to measure intracellular parameters on living cells in perfusion, with controlled medium.

Ethernet and USB ports are available for computer connection, and in conformity with SAFAS exclusive Sustainable SpectroscopyTM concept, software updates are free of charge for instrument''s life, the Xenon lamp has VERY long life, and spare parts are available for very long time. An innovative Web Service communication is available for easy connection to a robot for HTS.

Cost-effective and reliable, the Xenius is extremely evolutive, and basic versions are affordable even for very low budgets…